Our technology

Plus Lab employed and refined two technologies, namely, special high-temperature firing and original melting technology to develop BiSCaO Water.
These technologies are refined from many years of joint research and development by many researchers and research institutes, including a Professor Emeritus of National Defense Medical College who is now Plus Lab’s Chief Scientific Officer (CSO), and MI + Research Institute Hachinohe Lab.

The original research goal was to try to detoxify biochemical weapons, drugs, pesticides, toxic gases, etc., that is, to neutralize those toxic chemicals and gasses to make them friendly to humans, animals and the natural environment. In the course of research, Plus Lab found that BiSCaO Water has a powerful effect on harmful microbes such as viruses, bacteria and fungi. The incredible efficacy of BiSCaO Water was subsequently scientifically tested and confirmed by independent authorities.

BiSCaO Water is calcium ionized water (Ca2+). Its “high efficacy”, "high functionality" and "high safety" are attracting attention in various academic institutions in Japan and overseas.

Scientific tests

removal
test
01
Virus removal test

Despite its natural environment-friendly ingredient, BiSCaO Water is a powerful disinfectant and deodorant with excellent virus removal properties. It has been confirmed that the effect is equal to or better than products made with other active ingredients.
* Japan Food Research Laboratories (JFRL) / No.20027366001
Test virus 1 minute later 5 minutes later 15 minutes later
Feline calicivirus (norovirus) Not detected
-
-
Influenza virus 95% reduction Not detected
-
Test virus Feline calicivirus (norovirus)
1 minute later Not detected
5 minutes later
-
15 minutes later
-
Test virus Influenza virus
1 minute later 95% reduction
5 minutes later Not detected
15 minutes later
-
Japan Food Research Laboratories (JFRL) Report
Virus Inactivation Test
JFRL exam report
JFRL result report

removal
test
02
Sterilization comparative test

As a result of comparison with other companies' products, it was found that BiSCao Water has high sterilizing power.
Since it is a raw material that is kind to humans and the natural environment, it has been found that BiSCaO Water is very effective in disinfecting face masks and it does not compromise the integrity of woven and non-woven masks’ fabric and filters, unlike other products.
* Japan Food Research Laboratories (JFRL) / No.20027371001-0101

Comparison of BiSCaO Water with other disinfectants

8
Lots of bacteria and virus
7
6
5
4
3
2
1
0
Aseptic
Control
Undiluted
solution
2-fold
dilution
4-fold
dilution
Log
10
CFU(Colony Forming Unit)/ml
BiSCaO Water
Not detected
Not detected
8
Lots of bacteria and virus
7
6
5
4
3
2
1
0
Aseptic
Control
Undiluted
solution
2-fold
dilution
4-fold
dilution
Log
10
CFU(Colony Forming Unit)/ml
Sodium hypochlorite
Not detected
Not detected
100ppm
8
Lots of bacteria and virus
7
6
5
4
3
2
1
0
Aseptic
Control
Undiluted
solution
2-fold
dilution
4-fold
dilution
Log
10
CFU(Colony Forming Unit)/ml
Alcohol
100%
concentrate
50%
concentrate
25%
concentrate
8
Lots of bacteria and virus
7
6
5
4
3
2
1
0
Aseptic
Control
Undiluted
solution
2-fold
dilution
4-fold
dilution
Log
10
CFU(Colony Forming Unit)/ml
Povidone iodine
400%
concentrate
200%
concentrate
100%
concentrate

BiSCaO Water hand sanitizing test

Before sanitizing
hands before sanitizing
arrow to right
After sanitizing with BiSCaO Water
hands after sanitizing
BiSCaO Water is very kind to your skin and hands.

Bactericidal effect of BiSCaO Water on mask

6
Lots of bacteria and virus
5
4
3
2
1
0
Aseptic
Control
Spray
before use
Spray before
and after use
Log
10
CFU(Colony Forming Unit)/ml
General bactericidal
Not detected
Not detected
6
Lots of bacteria and virus
5
4
3
2
1
0
Aseptic
Control
Spray
before use
Spray before
and after use
Log
10
CFU(Colony Forming Unit)/ml
Coliforms
Not detected
Not detected
Prevents skin rash caused by wearing a mask for a long period of time
skin with P.acnes
Wearing a mask not sanitized with BiSCaO Water
Bacteria and viruses accumulate in the face mask from sweat and steam and from your breath when the mask is worn for some time. The accumulated sweat and steam make the mask smell unpleasant and can cause rashes and redness to your skin.
skin with no P.acnes
Wearing a mask sanitized with BiSCaO Water
BiSCaO Water kills bacteria and deodorizes the face mask making it smell fresher and cleaner when you wear it again. Unlike other sanitizers, BiSCaO Water also inactivates endotoxins which is a toxic by-product of dead germs and viruses. As a result, you can safely wear the face mask after spraying BiSCaO Water onto the inside of your face mask. BiSCaO Water does not irritate your skin. In fact, it is kind to your skin,

Bactericidal effect test

Coliforms
Test virus
1 minute later
5 minutes later
15 minutes later
Unprocessed
15 minutes later
Coliforms
60% reduction
Not detected no bacteria
-

full of bacteria
Coliforms (O157:H7)
Test virus
1 minute later
5 minutes later
15 minutes later
Unprocessed
15 minutes later
Coliforms (O157:H7)
10% reduction
99% reduction
Not detected no bacteria

full of bacteria
Pseudomonas aeruginosa
Test virus
1 minute later
5 minutes later
15 minutes later
Unprocessed
15 minutes later
Pseudomonas aeruginosa
90% reduction
Not detected no bacteria
-

full of bacteria
Salmonella enterica
Test virus
1 minute later
5 minutes later
15 minutes later
Unprocessed
15 minutes later
Salmonella enterica
90% reduction
Not detected no bacteria
-

full of bacteria
Staphylococcus aureus
Test virus
1 minute later
5 minutes later
15 minutes later
Unprocessed
15 minutes later
Staphylococcus aureus
65% reduction
99% reduction
Not detected no bacteria

full of bacteria
MRSA
Test virus
1 minute later
5 minutes later
15 minutes later
Unprocessed
15 minutes later
MRSA
55% reduction
70% reduction
99% reduction no bacteria

full of bacteria
Japan Food Research Laboratories (JFRL) Report
Virus Inactivation Test
JFRL exam report
JFRL result report

removal
test
03
Deodorant comparison test

BiSCaO Water has amazing odour removal (“deodorant”) power. The difference in deodorant effect between BiSCaO Water and products made with other active ingredients is very clear.
[Reference: Peer review paper - Molecules, 2020, 25 3001]
Nakamura, S., Ishihara, M., et al. Concentrated bioshell calcium oxide (BiSCaO) water kills pathogenic microbes: characterization and activity.

Deodorant comparison between BiSCaO Water and other companies' products

1400
Strong smell
1200
1000
800
600
400
200
0
Odorless
Control
Undiluted
solution
2-fold
dilution
4-fold
dilution
Odor
BiSCaO Water
1400
Strong smell
1200
1000
800
600
400
200
0
Odorless
Control
Undiluted
solution
2-fold
dilution
4-fold
dilution
Odor
Sodium hypochlorite
400ppm
200ppm
100ppm
1400
Strong smell
1200
1000
800
600
400
200
0
Odorless
Control
Undiluted
solution
2-fold
dilution
4-fold
dilution
Odor
Alcohol
100%
concentrate
50%
concentrate
25%
concentrate
1400
Strong smell
1200
1000
800
600
400
200
0
Odorless
Control
Undiluted
solution
2-fold
dilution
4-fold
dilution
Odor
Povidone iodine
400%
concentrate
200%
concentrate
100%
concentrate